All good things. . . this is the fifth and final Build-a-Gene of the series.
Synthetic biology offers scientists an unprecedented ability to manipulate living organisms. Beyond simply giving cells one or two new functions, scientists are now able to redesign and synthesize an organism’s entire genome. This has led both critics and advocates to claim that scientists are now able to “create life” in the lab.
These sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to go to all the sessions to enjoy the experience. There are no prerequisites!
This time we will screen the bacterial colonies by colony PCR to determine which ones contain a plasmid insert that is the correct size for the emGFP+promoter. We will verify this using gel electrophoresis. We will then discuss the last step of verification, sequencing to verify that you actually have the emGFP gene and promoter without any sequence errors.
Instructor: Dr. Lisa Scheifele
Cost of 5th session registration $50/ free for members.
We have background material on this at our Open Wetware Site
Here is an account of the series so far, from one of our newest members Dave Gray.
SO what are you waiting for Register Here
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