Synthetic biology offers scientists an unprecedented ability to manipulate living organisms. Beyond simply giving cells one or two new functions, scientists are now able to redesign and synthesize an organism’s entire genome. This has led both critics and advocates to claim that scientists are now able to “create life” in the lab.
In this 5-session “Build-a-Gene” class, we will learn and practice the techniques that synthetic biologists use to synthesize novel genes and genomes. We will design and synthesize one gene, a variant of the green fluorescent protein that will make our cells glow green. We will discuss how this technology is currently being used to re-design bacterial cells and create custom vaccines, and we will discuss how and by whom the use of this technology should be regulated. Join us as we learn to synthesize new genes from scratch!
These sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all the sessions to enjoy the experience. There are no prerequisites!
This time we will ligate our vector, the promoter, and the emGFP gene together into one circular DNA molecule. We will then do a transformation, where we place this DNA into a bacteria, where it will be replicated many times, as the cells grow.
You can also take a look at background material at our Open Wetware site.
The remaining sessions will be on Saturdays, August 17th and 24th from 9:00 AM to 1 PM
Instructor: Dr. Lisa Scheifele
Cost of 4th session: Early registration $50/ free for members until August 16th. Starting August 16th, cost is $60/ $10 for members (this does not include eventbrite fees) Please REGISTER HERE
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