Synthetic biology offers scientists an unprecedented ability to manipulate living organisms. Beyond simply giving cells one or two new functions, scientists are now able to redesign and synthesize an organism’s entire genome. This has led both critics and advocates to claim that scientists are now able to “create life” in the lab.
In this 5-session “Build-a-Gene” class, we will learn and practice the techniques that synthetic biologists use to synthesize novel genes and genomes. We will design and synthesize one gene, a variant of the green fluorescent protein that will make our cells glow green. We will discuss how this technology is currently being used to re-design bacterial cells and create custom vaccines, and we will discuss how and by whom the use of this technology should be regulated. Join us as we learn to synthesize new genes from scratch!
These sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all the sessions to enjoy the experience. There are no prerequisites!
This week we will be checking to see whether our emGFP gene was successfully assembled and amplified and then we will be purifying our promoter, vector and coding sequence. Once they are purified, we will cut the promoter, vector and coding sequence with restriction enzymes so that the three pieces can be joined together and transferred into E. coli cells in our next session.
The remaining sessions will be on Saturdays, August 10, 17, 24 all at 9:00 AM to 1 PM
Instructor: Dr. Lisa Scheifele
Cost of 3rd session: Early registration $50/ free for members until July 26. Starting July 26, cost is $60/ $10 for members (this does not include eventbrite fees) Please register HERE.
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