The final session of this course, walking you through the various ways in which genes can be isolated and cloned into bacteria and yeast, providing you with a foundation on the techniques of synthetic biology. The lab project portion of the course entails isolation and cloning of the gene encoding the Taq polymerase protein, and you will go home with the skills to make your own Taq polymerase, the enzyme at the heart of PCR.
We discussed the Polymerase Chain Reaction (PCR), introducing primer design using online resources, and isolated DNA from some thermus aquaticus that was grown up in the incubator. We have gone through the process of cloning the taq polymerase gene using PCR. In the last session, we checked the products using gel electrophoresis. In this final session we will continue with some protein electrophoresis and will do bacterial transformation using the Taq vector.
These five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all of the sessions, each session can stand on its own. You do not need to go to all five sessions to enjoy the experience. There are no prerequisites except interest!
Cost to register for this session is $65. Free to members. (Yes, minors can participate, under the supervision of an adult.) Please register HERE
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