How to clone a gene! This five session course will walk you through the various ways in which genes can be isolated and cloned into bacteria and yeast, providing you with a foundation on the techniques of synthetic biology.
The lab project portion of the course will entail isolation and cloning of the gene encoding the Taq polymerase protein, and you will go home with the skills to make your own Taq polymerase – the enzyme at the heart of PCR, whose virtues are hailed by synthetic biologists everywhere, especially when sponsored by certain multi-billion dollar companies. . .
Along the way you’ll learn how to isolate DNA, how to use freely available software to design and check cloning projects, how to design primers for PCR projects, how to set up and run a PCR reaction, How to digest DNA with restriction enzymes, how to ligate DNA and create recombinant DNA molecules, how to transform bacteria and identify those that contain your recombinant DNA, and lastly how to assay your recombinant molecule for activity.
The five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all five sessions to enjoy the experience. There are no prerequisites except interest!
Sessions are all on Saturdays at 10:00 am to about 1:00 pm, on Feb 1, Feb 15, March 1, March 15th, and March 22
Cost to register for this first session is $65. Free to members. Please register HERE
Please feel free to email us with questions at firstname.lastname@example.org
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