Build-a-BUG 5: Yeast Spore Mating Type Detector Saturday April 6 noon to 4:00 pm
Learn the fundamentals of synthetic biology in eukaryotes! We will use BioBrick standard biological parts to engineer Saccharomyces cerevisiae, a classic model organism and the yeast commonly used in winemaking, baking, and brewing. This yeast can be induced to form spores, which are daughter cells with half the number of chromosomes. Similar to the sperm and egg cells of male and female animals, these spores can be one
of two mating types: a or α. An a spore can mate with an α spore to form a new yeast cell. The goal will be to build a yeast that when sporulated will yield mating type α spores that fluoresce red. This project has potential real-world biotechnology applications (e.g. streamlining the identification of spores for downstream mating experiments).
This is the fifth Build-A-BUG workshop in a series of five (or possibly six) on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, After successful ligations and E. coli transformations in Session 4, some students have verified their constructs with colony PCR; they then grew up and isolated the pDNA. We will now conclude this Build-a-BUG series by transforming S. cerevisae (yeast) cells with our mating type detector pDNA constructs.
The five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all five sessions to enjoy the experience. There are no prerequisites, except you must be over 18 years of age (still working on legal stuff to let younger people participate in this activity)
Also, we now have a repository wiki on Open Wet Ware that provides notes, background material, and results for these sessions.
Instructor: Dileep Monie
Cost: Early registration $40/ free for members until April 5. Starting April 5, cost is $50/ $10 for members
Please Register HERE!
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