Build-a-BUG 1: Yeast Spore Mating Type Detector Saturday February 9 noon to 4:00 pm
Learn the fundamentals of synthetic biology in eukaryotes! We will use BioBrick standard biological parts to engineer Saccharomyces cerevisiae, a classic model organism and the yeast commonly used in winemaking, baking, and brewing. This yeast can be induced to form spores, which are daughter cells with half the number of chromosomes. Similar to the sperm and egg cells of male and female animals, these spores can be one
of two mating types: a or α. An a spore can mate with an α spore to form a new yeast cell. The goal will be to build a yeast that when sporulated will yield mating type α spores that fluoresce red. This project has potential real-world biotechnology applications (e.g. streamlining the identification of spores for downstream mating experiments).
This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project by doing minipreps (DNA isolation), restriction digests (cutting DNA), and agarose gel electrophoresis (visualizing DNA). Other Build-A-BUG workshops in this series will cover other techniques.
The five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all five sessions to enjoy the experience. There are no prerequisites, except you must be over 18 years of age (still working on legal stuff to let younger people participate in this activity)
The entire series will be on Saturdays 2/9, 2/23, 3/9, 3/23, and 4/6; noon to 4 PM
Instructor: Dileep Monie
Cost: $40 per session (+ eventbrite fees)/ free for members Please register HERE
Join us this (and every) Thursday from 7-9pm and get your Bio-Art and Philosophy on!
Find out more about what exactly Synthetic Biology is, and how it can be used to construct art.
From Bio-Couture (http://www.youtube.com/watch?v=WVW-jSdhILs), to Bio-Fiction (http://bio-fiction.com/en/), from ArtScience (http://www.artscienceprize.org/asp/what-is-artscience), to Synthetic Aesthetics (http://www.syntheticaesthetics.org/), and E. Musici (bacteria that are designed to move and create sound: http://2012.igem.org/Team:USC), to creating a Blood Lamp (a statement about our energy consumption: http://www.miket.co.uk/blood_lamp.html). Let’s have some fun and create a space in Baltimore where artists and citizen scientists can collaborate and make works of bio-art and design together.
BUGSS All Ages Open House Event 02/02/13
Certain fruits, like strawberries, provide an exceptional way to demonstrate basic DNA techniques. At the BUGSS All Ages Open House Event this Saturday February 2nd 2013, kids of all ages will be able to complete a DNA extraction and see first hand that DNA is in the food you eat. Each person will learn the simple method to extract DNA from fruit and why each step is necessary due to the complex organization of DNA in cells.
This event is open to the public free of charge from 11-3pm.
There will be a MudWatt™ Microbial Fuel Cell (MFC) Kit demonstration, an art station set up for everyone to make bio-inspired crafts, light food and beverages, and more! Stop by with the whole family and see what BUGSS is all about!
BUGSS is a 501(C)3 charity. Please help keep BUGSS going!